MSCI 935 - Techniques II - Methods for Nucleic Acids

Cr Hrs: 2 (32-0-0)
GR

The theory and practical application of commonly used techniques for working with nucleic acids are considered, including nucleic acid isolation, quantitation, and electrophoresis; hybridization, mini-and micro arrays; DNA sequencing; oligonucleotide synthesis, uses, PCR and qPCR; restriction and modification enzymes, and polymerases; prokaryotic and eukaryotic cloning vectors; library construction and clone detection; cDNA-expression and siRNA-silencing libraries; making transgenic mice; in vitro mutagenesis, gel retardation, footprinting, and chromatin assays.

Grade Mode: Standard

Mode of Delivery (Online or Hybrid): Hybrid
Instructional Method: Lecture

Prerequisites: Limited to students who have completed “Biochemistry” or “Cell biology”
Term offered: Fall

Fall - Instructor of Record: Laribee, Ronald N

Fall - Additional Instructor 1: Fan, Meiyun